The First Edition of “EAACI Molecular Allergology User’s Guide” was released in 2016 and rapidly became a key reference for clinical and basic scientists. However, knowledge on additional allergen sources, on the role of co-factors, and underlying molecular mechanisms has expanded since then.

Therefore, a second edition was developed. With the contribution from more than 95 authors, the content was revised and new allergen sources and allergen protein families included.

The new Molecular Allergology User’s Guide 2.0 provides state-of-the-art information on allergen molecules, their clinical relevance and application in diagnostic algorithms for clinical practice. It is designed for both, clinicians and scientists, guiding health care professionals through the overwhelming list of different allergen molecules identified available for testing. Further, it provides diagnostic algorithms based on current knowledge about the clinical relevance of allergen molecules and gives an overview on the basic mechanisms of test formats, the biology of allergen molecules and the application of tests to measure allergen exposure.